Laser, as an emerging artificial light source, is one of the greatest inventions of the 20th century, featuring high power density, excellent directionality, and superb monochromaticity. It has been widely applied in the agricultural sector. This article provides an in-depth analysis of the research progress and development trends of laser technology in agriculture. In plant production, it discusses applications in plant mutation breeding, promoting plant growth and development, enhancing yield and quality, plant protection, plant detection, and phenotyping. In animal management, the focus is on the use of laser technology in animal genetic breeding, growth, medical care, and product testing. For fungal research, it summarizes the use of laser technology in fungal breeding, growth, detection, and identification. The article also addresses the challenges and difficulties faced when integrating laser technology with agricultural production, proposing directions for future development, such as improving precision in plant production, optimizing animal management, and advancing scientific fungal production. Although laser technology has already achieved significant results in agriculture, future research should explore more innovative applications and integrate with artificial intelligence and big data technologies to further advance the deep integration of laser technology with the agricultural industry, leading to more efficient and intelligent agricultural production management and offering new opportunities and breakthroughs for modern agriculture.